Canine Diabetes Wiki
Winthrop Basal
NPH/Isophane by Aventis
U40, U100 isophane
Action in dogs:
  • onset 0.5-3h,
  • peak 2-10h, [1]
  • duration 4-24h [2]
Line: Winthrop
Also known as: Insuhuman Basal, Insuman Basal, Isuhuman Basal
Similar to: Humulin I, Huminsulin Basal, Humulin N, Humulin NPH, Humulina NPH, Humuline NPH, Humuline N, Umuline NPH, Humulin ReliOn N
Insulatard, Novolin N, Protaphan, Protaphane
ReliOn/Novolin NPH

Names of Lilly r-DNA/GE/GM insulins worldwide
Use and Handling:
Shelf Life: 24 months Type: cloudy
When Opened: 4 weeks room temp.
In Pen: 4 weeks room temp.
Notes: Protect from light and heat
Do Not Freeze, Resuspend
Do not use if product does not re-suspend
Do not use intravenously [3]

Insuman insulins in pen cartridge form.

Note that some countries have the brand name in U40 strength.

This is another of Aventis' brand name [4] for its NPH/isophane suspension insulin, [5][6][7] which is intermediate-acting and identical to the Insuman line. [4][8]

Both Novo Nordisk and Eli Lilly produce similar insulins under the names Novolin N, [9] Insulatard, [10] Protaphane, [11] Humulin N, [12][13] Umuline NPH, Humulina NPH, Humuline NPH, Huminsulin Basal and Humulin I. [14][15][16]

Like all other isophane/protamine suspended insulins, it is a crystalline suspension with protamine.

This type of insulin tends to have an onset of 2-4 hours, a peak action of 4-10 hours, and a total duration of 10-20 hours, in humans.

Specifications of NPH/isophane insulin[]

British National Formulary [17] defines this type as: A sterile suspension bovine or porcine or of human insulin in the form of a complex obtained by the addition of protamine sulphate or another suitable protamine.

British Pharmacoepia and United States Pharmacopia definitions: [18]

Isophane Insulin

Sterile buffered suspension of insulin in the form of a complex obtained by addition of suitable protamine. Prepared from crystalline insulin. pH 6.9 - 7.5 iso-osmotic with blood. Contains for each 100 units of insulin, 300 - 600 g protamine sulphate and not more than 40 g zinc, a suitable bactericide and sodium phosphate as buffering agent.

USP specification: Sterile suspension of zinc insulin crystalline and protamine sulphate in buffered water for injection. Solid phase contain crystals of insulin protamine and zinc; 40, 80, 100 units/ml. Contains glycerol, metacresol, phenol sodium phosphate and zinc.

NPH/isophane insulins[]

The following commercial insulins are all generically NPH insulins:

Other commonly used names are:

  • NPH insulin, NPH
  • isophane insulin


  1. Insulins. North American Veterinary Conference (2005).
  2. Ruchinsky, Renee, et. al. (2010). Diabetes Management Guidelines for Dogs and Cats-page 7. American Animal Hospital Association.
  3. Maddison, Jill E.,Page, Stephen W.,Church, David B. (2008). Small Animal Clinical Pharmacology. Saunders Ltd..
  4. 4.0 4.1 Winthrop Insulins. EMEA. Cite error: Invalid <ref> tag; name "Winthrop" defined multiple times with different content
  5. Patient Information Leaflet-Insuman Basal. Sanofi-Aventis.
  6. Insuman Prescribing Information.
  7. Aventis Poster-Insuman & Lantus Insulins. Sanofi-Aventis.
  8. Scientific Discussion-Insuman. EMEA.
  9. Novolin N. Revolution Health.
  10. Scientific Discussion-Insulatard. EMEA.
  11. Scientific Discussion-Protaphane. EMEA.
  12. Humulin N. Eli Lilly.
  13. Humulin N NPH/Isophane Human Insulin. Revolution Health.
  14. Patient Information Leaflet-Humulin I. Eli Lilly.
  15. Humulin I Prescribing Information.
  16. Lilly EU/UK Product Sheet & Time Activity Profiles. Eli Lilly.
  17. British National Formulary-Isophane/NPH Insulin Definition. InChem.
  18. British Pharmacoepia (BP) & United States Pharmacoepia (USP)-Isophane/NPH Insulin Defined. InChem.
  19. Hypurin Bovine Isophane Patient Information. Net Doctor UK.
  20. Hypurin Bovine Isophane. Net Doctor UK.
  21. Prescribing Bovine Insulin.
  22. 22.0 22.1 Response to Withdrawal of Novo Porcine Insulins. CP/Wockhardt UK.
  23. Prescribing Porcine Insulin.
  24. Prescribing Novo Animal Insulin.
  25. Patient Information Leaflet-Pork Insulatard. Novo Nordisk.

More Information[]

Related Pages
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Humulin I
Humulin N
Novolin N
Insuman Basal