Canine Diabetes Wiki
Novolin N
NPH/Isophane by Novo Nordisk
U100 isophane
Action in dogs:
  • onset 0.5-3h,
  • peak 2-10h, [1]
  • duration 4-24h [2]
Line: Novolin
Also known as: Insulatard, Protaphan,
Protaphane,  ReliOn/Novolin NPH
Similar to: Humulin I, Huminsulin Basal, Humulin N, Humulin NPH, Humulina NPH, Humuline NPH, Humuline N, Umuline NPH, Humulin ReliOn N
Insuhuman Basal, Winthrop Basal
Insuman Basal, Isuhuman Basal

Names of Lilly r-DNA/GE/GM insulins worldwide
Use and Handling:
Shelf Life: 18 months Type: cloudy
When Opened: 6 weeks room temp.
In Pen: 6 weeks room temp.
Notes: Protect from light and heat
Do Not Freeze, Resuspend
Do not use if product does not re-suspend
Do not use intravenously [3]

NVN34740 72167 5

Novolin NPH/isophane insulin.

Novo Nordisk's intermediate-acting human insulin, known in some countries as Insulatard [4] or Protaphane. [5]

As of January 1, 2010, Novo will discontinue offering Novolin N in cartridges for refillable insulin pens. [6][7]

This type of insulin tends to have an onset of 2-4 hours, a peak action of 4-10 hours, and a total duration of 10-20 hours, in humans.

Like Humulin N, [8][9] it is an isophane crystal protamine suspension insulin. [10]

Other brands are: Humulin I, [11][12][13] Umuline NPH, Humulina NPH, Humuline NPH, and Huminsulin Basal by Eli Lilly and Insuman Basal [14][15] and Winthrop Basal by Aventis.

Specifications of NPH/isophane insulin[]

British National Formulary [16] defines this type as: A sterile suspension bovine or porcine or of human insulin in the form of a complex obtained by the addition of protamine sulphate or another suitable protamine.

British Pharmacoepia and United States Pharmacopia definitions: [17]

Isophane Insulin

Sterile buffered suspension of insulin in the form of a complex obtained by addition of suitable protamine. Prepared from crystalline insulin. pH 6.9 - 7.5 iso-osmotic with blood. Contains for each 100 units of insulin, 300 - 600 g protamine sulphate and not more than 40 g zinc, a suitable bactericide and sodium phosphate as buffering agent.

USP specification: Sterile suspension of zinc insulin crystalline and protamine sulphate in buffered water for injection. Solid phase contain crystals of insulin protamine and zinc; 40, 80, 100 units/ml. Contains glycerol, metacresol, phenol sodium phosphate and zinc.

NPH/isophane insulins[]

The following commercial insulins are all generically NPH insulins:

Other commonly used names are:

  • NPH insulin, NPH
  • isophane insulin


  1. Insulins. North American Veterinary Conference (2005).
  2. Ruchinsky, Renee, et. al. (2010). Diabetes Management Guidelines for Dogs and Cats-page 7. American Animal Hospital Association.
  3. Maddison, Jill E.,Page, Stephen W.,Church, David B. (2008). Small Animal Clinical Pharmacology. Saunders Ltd..
  4. Prescribing Novo Insulins.
  5. Scientific Discussion-Protaphane. EMEA.
  6. Novo discontinuation notice for pens. Novo Nordisk.
  7. Novolin N cartridges Discontinuation Notice. US Food and Drug Administration.
  8. Humulin N patient leaflet. Eli Lilly.
  9. Humulin N NPH/Isophane Insulin. Revolution Health.
  10. Scientific Discussion-Insulatard. EMEA.
  11. Humulin I Prescribing Information.
  12. Patient Information Leaflet-Humulin I. Eli Lilly.
  13. Lilly EU/UK Product Sheet & Time Activity Profiles. Eli Lilly.
  14. Patient Information Leaflet-Insuman Basal. Sanofi-Aventis.
  15. Insuman Prescribing Information.
  16. British National Formulary-Isophane/NPH Insulin Definition. InChem.
  17. British Pharmacoepia (BP) & United States Pharmacoepia (USP)-Isophane/NPH Insulin Defined. InChem.
  18. Hypurin Bovine Isophane Patient Information. Net Doctor uk.
  19. Hypurin Bovine Isophane. Wockhardt UK.
  20. Prescribing Bovine Insulin.
  21. 21.0 21.1 Response to Withdrawal of Novo Porcine Insulins. CP/Wockhardt UK. Cite error: Invalid <ref> tag; name "Wockhardt" defined multiple times with different content
  22. Prescribing Porcine Insulin.
  23. Prescribing Novo Animal Insulin.
  24. Patient Information Leaflet-Pork Insulatard. Novo Nordisk.

More Information[]

Related Pages
Humulin n 21938f NPHtap DSC02817 Insulatard Protaphan2
Humulin N
Humulin I
Humulin ReliOn N
Insuman Basal