Novolin N
NPH/Isophane by Novo Nordisk
U100 isophane
Action in dogs:
  • onset 0.5-3h,
  • peak 2-10h, [1]
  • duration 4-24h [2]
Line: Novolin
Also known as: Insulatard, Protaphan,
Protaphane,  ReliOn/Novolin NPH
Similar to: Humulin I, Huminsulin Basal, Humulin N, Humulin NPH, Humulina NPH, Humuline NPH, Humuline N, Umuline NPH, Humulin ReliOn N
Insuhuman Basal, Winthrop Basal
Insuman Basal, Isuhuman Basal

Names of Lilly r-DNA/GE/GM insulins worldwide
Use and Handling:
Shelf Life: 18 months Type: cloudy
When Opened: 6 weeks room temp.
In Pen: 6 weeks room temp.
Notes: Protect from light and heat
Do Not Freeze, Resuspend
Do not use if product does not re-suspend
Do not use intravenously [3]

NVN34740 72167 5

Novolin NPH/isophane insulin.

Novo Nordisk's intermediate-acting human insulin, known in some countries as Insulatard [4] or Protaphane. [5]

As of January 1, 2010, Novo will discontinue offering Novolin N in cartridges for refillable insulin pens. [6][7]

This type of insulin tends to have an onset of 2-4 hours, a peak action of 4-10 hours, and a total duration of 10-20 hours, in humans.

Like Humulin N, [8][9] it is an isophane crystal protamine suspension insulin. [10]

Other brands are: Humulin I, [11][12][13] Umuline NPH, Humulina NPH, Humuline NPH, and Huminsulin Basal by Eli Lilly and Insuman Basal [14][15] and Winthrop Basal by Aventis.

Specifications of NPH/isophane insulinEdit

British National Formulary [16] defines this type as: A sterile suspension bovine or porcine or of human insulin in the form of a complex obtained by the addition of protamine sulphate or another suitable protamine.

British Pharmacoepia and United States Pharmacopia definitions: [17]

Isophane Insulin

Sterile buffered suspension of insulin in the form of a complex obtained by addition of suitable protamine. Prepared from crystalline insulin. pH 6.9 - 7.5 iso-osmotic with blood. Contains for each 100 units of insulin, 300 - 600 g protamine sulphate and not more than 40 g zinc, a suitable bactericide and sodium phosphate as buffering agent.

USP specification: Sterile suspension of zinc insulin crystalline and protamine sulphate in buffered water for injection. Solid phase contain crystals of insulin protamine and zinc; 40, 80, 100 units/ml. Contains glycerol, metacresol, phenol sodium phosphate and zinc.

NPH/isophane insulinsEdit

The following commercial insulins are all generically NPH insulins:

Other commonly used names are:

  • NPH insulin, NPH
  • isophane insulin


More InformationEdit

Related Pages
Humulin n 21938f NPHtap DSC02817 Insulatard Protaphan2
Humulin N
Humulin I
Humulin ReliOn N
Insuman Basal
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