Canine Diabetes Wiki
Mixtard 20
R/Neutral/NPH/Isophane Mix by Novo Nordisk
U100 isophane
Action in dogs:
  • onset 0.5-3h,
  • peak 2-10h,
  • duration 4-24h
Line: Novo
Also known as: Actraphane 20/80, Penmix 20
Similar to: Huminsulin Profil II, Humulin 20/80,
Humulina 20/80, Humuline 20/80, Humulin M2,
Profil II, Humulin M, Umuline Profil 20, Profil 20
Humulin 80/20

Names of Lilly r-DNA/GE/GM insulins worldwide
Use and Handling:
Shelf Life: 30 months Type: cloudy
When Opened: 6 weeks room temp.
In Pen: 6 weeks room temp.
Notes: Protect from light and heat
Do Not Freeze, Resuspend
Do not use if product does not re-suspend
Do not use intravenously [1]
Penfill Mixtard 20 1

3 ml pen cartridge of Novo's Mixtard 20.

Note that some countries have the brand name in U40 strength.

A mixed, intermediate-acting insulin [2] made by Novo Nordisk containing 20% R/neutral insulin and 80% NPH/isophane insulin crystals. [3]

Comparable product is also made by Novo: Actraphane 20/80. [4]

Eli Lilly once produced a similar insulin in the EU & UK called Humulin M2; [5] it was discontinued in July 2004. [6] Their comparable insulin sold in North America, Humulin 20/80, is also no longer available. The insulin is also known by Umuline Profil 20, Huminsulin Profil II, Humuline 20/80, Humulina 20/80, and in some places, Humulin M.

Mixtard 20 was discontinued December 31, 2007. [7][8]

Mixed insulins cover a wide range as they can be animal origin, r-DNA/GE/GM origin or analog insulins. The non-analog mixed insulins are made up of intermediate-acting NPH/isophane insulin and short-acting R/neutral. normal insulin. The analog mixes are made from intermediate-acting protamine-suspended analog insulin (suspended similar to NPH/isophane insulin) and fast-acting analog insulin. They can also vary in the fraction (amount or percent) of the two types (R/neutral and NPH/isophane) of insulin used to make a non-analog mix, and in the percentage of fast/rapid-acting analog insulin and slower-acting protamine suspended analog insulin.

For some reason, those in the US choose to list the slower, NPH/isophane based insulin fraction first, such as Humulin or Novolin 70/30. The rest of the world gives the faster insulin top billing and the result is 30/70. Regardless of how this is written, it means that the insulin is made up of 70% NPH/isophane insulin and 30% R/neutral insulin.

The most common mixes are 70/30 (30/70) (non analog) or 75/25 (25/75) (analog). There are no currently-marketed mixes which combine insulins of different origins. Eli Lilly's form of beef/pork Iletin and Novo Nordisk's Lentard were discontinued some time ago. Here are the most common ones that could cause confusion:


Related pages
20 80
20 80
20 80
Humulin M2
Penmix 20
Actraphane 20/80
Humulin 20/80
Humulin M2