Canine Diabetes Wiki
Hypurin Vet Protamine Zinc
PZI by CP Pharma
U100 protamine
Action in dogs:
  • onset 8-20h,
  • peak Xh,
  • duration Xh rarely used [1][2]
Line: Hypurin
Also known as: Hypurin Bovine Protamine Zinc, Beef PZI
Similar to: Insuvet Protamine Zinc, VOPA PZI
Use and Handling:
Shelf Life: 36 months Type: cloudy
When Opened: 28 days room temp.
In Pen: N/A
Notes: Protect from light and heat
Do Not Freeze, store at 2-8C, Resuspend
Do not use if product does not re-suspend 
Do not use intravenously [3]
Intramuscular use is not advised
Do not mix Protamine Zinc insulin in the same syringe with R/Neutral insulin
Cp wockhardt bovine insulins

Hypurin Bovine insulins-Protamine Zinc is in the middle with an orange cap.

Hypurin Vet Protamine Zinc [4] was a beef-based PZI insulin [5] made by CP Pharma. [6]

It was approved only for veterinary use and for distribution within the UK --the human-approved equivalent, Hypurin Bovine Protamine Zinc is still available; the comparable Insuvet Protamine Zinc has been discontinued. [7][8][9] All are or were 100% bovine, U100 PZI.

US-compounded BCP PZI and Summit's PZI U100 strength insulin is comparable to this one.

NOAH Compendium-UK [10] officially describes the comparable Insuvet Protamine Zinc as highly purified bovine protamine zinc (PZI) insulin, according to British Pharmacopeia (BP) standards and definitions. It is U100 strength and presented in 10ml vials.

It is described [11] as being a medium to long lasting insulin, with onset from 2-5 hours after injection, peak at 12-24 hours, and maximum duration of 24-36 hours.

NOAH goes on to say that onset, peak and duration times have been reported as being variable in dogs.

Technical details[]

PZI contains more zinc and protamine than NPH/isophane insulin and it is primarily the extra protamine which gives it its long-acting duration.[12] The additional protamine in the suspension [13] must first be broken down by proteolytic enzymes AKA proteases, [14] before the insulin in PZI can be absorbed. NPH/isophane insulin contains less protamine than PZI does and because there's less, it is an intermediate-acting insulin.

Usage and handling[]

Long acting

All PZI's are long-acting insulins with activity profiles similar to this.

Dosage information [15] for Insuvet Protamine Zinc PZI.

This insulin [16] insulin is to be given subcutaneously only.

Store at [17] 2-8C; do not freeze. Insuvet Protamine Zinc needs rolling. Product should not be used if it does not re-suspend. The vial has a life of 28 days after first use and a shelf life of 2 years [18].

Comparable insulins are: Hypurin Bovine Protamine Zinc, [19] which is approved for human use. The latter is the only one which can be exported from the UK. The U100 strength of BCP PZI and Summit PZI is also comparable to this insulin.

Insuvet PZI comes in a 10ML vial. It should be kept refrigerated and rolled gently several times before use to insure it is well-mixed and shows a uniform milky consistency.

No Combining For PZI

Protamine Zinc PZI [20] insulin cannot be combined with other insulins, due to the amount of protamine in the insulin's formula. [21][22][23][24]


  1. Insulins. North American Veterinary Conference-Clinicians Brief (2005).
  2. Scott-Moncrieff, Catherine (2009). Canine and Feline Diabetes Mellitus I-page 4. Western Veterinary Conference.
  3. Maddison, Jill E.,Page, Stephen W.,Church, David B. (2008). Small Animal Clinical Pharmacology. Saunders Ltd..
  4. Definition of Protamine Zinc Insulin. Merck Manual.
  5. Hypurin Bovine Protamine Zinc Patient Information. Net
  6. Hypurin Bovine Protamine Zinc. Wockhardt UK.
  7. Discontinuation of Insuvet Insulins. My Online Vet UK.
  8. Insuvet Discontinuation. VioVet UK.
  9. Pfizer insulin products currently unavailable. Veterinary News (29 October 2010).
  10. Insuvet. NOAH Compendium-UK.
  11. Insuvet-Uses. NOAH Compendium-UK.
  12. Greco, Deborah (2010). Treating Diabetes Mellitus in Dogs and Cats. Western Veterinary Conference.
  13. Williams, Robert (1968). PZI Insulin--More Protamine Than NPH/Isophane Insulin-Textbook of Endocrinology, 4th edition. W.B. Saunders.
  14. Proteolytic Enzymes. Wikipedia.
  15. Insuvet-Dosage & Administration. NOAH Compendium-UK.
  16. Insuvet-Contraindications/Warnings. NOAH Compendium-UK.
  17. Insuvet-Pharmaceutical Information. NOAH Compendium-UK.
  18. Hypurin Bovine Protamine Zinc. Wockhardt UK.
  19. Prescribing Bovine Insulin.
  20. DeRuiter, Jack. Excess Protamine in Protamine Zinc Insulin Formula. Auburn University.
  21. Harrower AD, Duncan LJ, Clarke BF. (1975). Comparison of Injection Techniques for Soluble and Protamine Zinc Insulins in Diabetes Mellitus. Practitioner.
  22. Protamine Zinc Insulin (PZI) Binds to Soluble Insulin in Same Syringe. NHS.
  23. Petsulin Protamine Zinc Page 5 Incompatibilities. Dechra.
  24. Inject Protamine Zinc Insulin Separately-Excess Protamine. Merck Manual.

Related pages
91 main
Cp wockhardt bovine insulins
Long acting
Insuvet Protamine Zinc
Hypurin Bovine Protamine Zinc
Pure Bovine PZI