Canine Diabetes Wiki
Hypurin Bovine Protamine Zinc
PZI by CP Pharma
U100 protamine
Action in dogs:
  • onset 8-20h,
  • peak Xh,
  • duration Xh rarely used [1][2]
Line: Hypurin
Also known as: Hypurin Vet Protamine Zinc, Beef PZI
Similar to: Insuvet Protamine Zinc, VOPA PZI
Use and Handling:
Shelf Life: 36 months Type: cloudy
When Opened: 28 days room temp.
In Pen: N/A
Notes: Protect from light and heat
Do Not Freeze, store at 2-8C, Resuspend
Do not use if product does not re-suspend 
Do not use intravenously [3]
Intramuscular use is not advised
Do not mix Protamine Zinc insulin in the same syringe with R/Neutral insulin.
69 main

Hypurin Bovine Protamine Zinc with its orange cap.

Hypurin Bovine Protamine Zinc [4] is a beef-based PZI insulin made by CP Pharma. [5][6][7]

Comparable insulins, made for veterinary use only are: the U100 strength insulins of BCP PZI and Summit. BCP PZI is compounded in the US, Summit in Canada; only CP Pharma's human-use approved insulin is able to be exported from the UK.For those who are in the UK, Best Pet Pharmacy has this in stock. Both Hypurin Vet Protamine Zinc and Insuvet Protamine Zinc are now discontinued.[8][9][10]

NOAH Compendium-UK [11] officially describes the comparable Insuvet Protamine Zinc as highly purified bovine protamine zinc (PZI) insulin, according to British Pharmacopeia (BP) standards and definitions. It is U100 strength and presented in 10ml vials. It is described [12] as being a medium to long lasting insulin, with onset from 2-5 hours after injection, peak at 12-24 hours, and maximum duration of 24-36 hours.

NOAH goes on to say that onset, peak and duration times have been reported as being variable in dogs.

Technical details[]

PZI contains more zinc and protamine than NPH/isophane insulin and it is primarily the extra protamine which gives it its long-acting duration.[13] The additional protamine in the suspension [14] must first be broken down by proteolytic enzymes AKA proteases, [15] before the insulin in PZI can be absorbed. NPH/isophane insulin contains less protamine than PZI does and because there's less, it is an intermediate-acting insulin.

Usage and handling[]

Long acting

All PZI's are long-acting insulins with activity profiles similar to this.

Dosage information [16] for Insuvet Protamine Zinc PZI.

This insulin [17] insulin is to be given subcutaneously only.

Store at [18]2-8C; do not freeze. Insuvet Protamine Zinc needs rolling. Product should not be used if it does not re-suspend. The vial has a life of 28 days after first use and a shelf life of 2 years. [6]

Insuvet PZI comes in a 10ML vial. It should be kept refrigerated and rolled gently several times before use to insure it is well-mixed and shows a uniform milky consistency.

No Combining For PZI

Protamine Zinc PZI [19] insulin cannot be combined with other insulins, due to the amount of protamine in the insulin's formula. [20][21][22][23]


  1. Insulins. North American Veterinary Conference-Clinicians Brief (2005).
  2. Scott-Moncrieff, Catherine (2009). Canine and Feline Diabetes Mellitus I-page 4. Western Veterinary Conference.
  3. Maddison, Jill E.,Page, Stephen W.,Church, David B. (2008). Small Animal Clinical Pharmacology. Saunders Ltd..
  4. Definition of Protamine Zinc Insulin. Merck Manual.
  5. Hypurin Bovine Protamine Zinc Patient Information. Net
  6. 6.0 6.1 Hypurin Bovine Protamine Zinc. Wockhardt UK. Cite error: Invalid <ref> tag; name "Wockhardt" defined multiple times with different content
  7. Prescribing Bovine Insulin.
  8. Discontinuation of Insuvet Insulins. My Online Vet UK.
  9. Insuvet Discontinuation. VioVet UK.
  10. Pfizer insulin products currently unavailable. Veterinary News (29 October 2010).
  11. Insuvet. NOAH Compendium-UK.
  12. Insuvet-Uses. NOAH Compendium-UK.
  13. Greco, Deborah (2010). Treating Diabetes Mellitus in Dogs and Cats. Western Veterinary Conference.
  14. Williams, Robert (1968). PZI Insulin--More Protamine Than NPH/Isophane Insulin-Textbook of Endocrinology, 4th edition. W.B. Saunders.
  15. Proteolytic Enzymes. Wikipedia.
  16. Insuvet-Dosage & Administration. NOAH Compendium-UK.
  17. Insuvet-Contraindications/Warnings. NOAH Compendium-UK.
  18. Insuvet-Pharmaceutical Information. NOAH Compendium-UK.
  19. DeRuiter, Jack. Excess Protamine in Protamine Zinc Insulin Formula. Auburn University.
  20. Harrower AD, Duncan LJ, Clarke BF. (1975). Comparison of Injection Techniques for Soluble and Protamine Zinc Insulins in Diabetes Mellitus. Practitioner.
  21. Protamine Zinc Insulin (PZI) Binds to Soluble Insulin in Same Syringe. NHS.
  22. Petsulin Protamine Zinc Page 5 Incompatibilities. Dechra.
  23. Inject Protamine Zinc Insulin Separately-Excess Protamine. Merck Manual.

Related pages
91 main
Long acting
Cp wockhardt bovine insulins
Insuvet Protamine Zinc
Pure Bovine PZI
Hypurin Vet Protamine Zinc