Canine Diabetes Wiki
Humuline 50/50
R/Neutral/NPH/Isophane Mix by Eli Lilly
U40, U100 isophane
Action in dogs:
  • onset 0.5-3h,
  • peak 2-10h,
  • duration 4-24h
Line: Humulin
Also known as: Humulin M5, Humulin 50/50,Huminsulin 50/50, Humulina 50/50
Similar to: Mixtard 50, Penmix 50, Actraphane 50/50
Insuhuman Comb 50, Insuman Comb 50
Winthrop Comb 50

Names of Lilly r-DNA/GE/GM insulins worldwide
Use and Handling:
Shelf Life: 24 months Type: cloudy
When Opened: 6 weeks room temp.
In Pen: 6 weeks room temp.
Notes: Protect from light and heat
Do Not Freeze, Resuspend
Do not use if product does not re-suspend
Do not use intravenously [1]
Wp comb50 1

Time activity profile for 50/50 insulin.

Humuline 50/50 is a brand name for Lilly's r-DNA/GE/GM human insulin mix consisting of 50% R/neutral insulin and 50% NPH/isophane crystal [2] insulin. [3][4] The US version of this insulin, Humulin 50/50, was recently discontinued by Lilly. It is also known as Humulina 50/50.

It is the same as Mixtard 50, [5] Actraphane 50/50 [6][7] and Aventis' Insuman Comb 50 [8][9] and Winthrop Comb 50.

Lilly stopped offering this insulin, under the brand name Humulin M5, [10] in the EU & UK in July 2004. [11][12]

Humulin 50/50 has been discontinued. This notice dated August 12, 2009, indicates there would be a supply of it until April 2010. [13][14][15]

Mixed insulins cover a wide range as they can be animal origin, r-DNA/GE/GM origin or analog insulins. The non-analog mixed insulins are made up of intermediate-acting NPH/isophane insulin and short-acting R/neutral. normal insulin. The analog mixes are made from intermediate-acting protamine-suspended analog insulin (suspended similar to NPH/isophane insulin) and fast-acting analog insulin. They can also vary in the fraction (amount or percent) of the two types (R/neutral and NPH/isophane) of insulin used to make a non-analog mix, and in the percentage of fast/rapid-acting analog insulin and slower-acting protamine suspended analog insulin.

For some reason, those in the US choose to list the slower, NPH/isophane based insulin fraction first, such as Humulin or Novolin 70/30. The rest of the world gives the faster insulin top billing and the result is 30/70. Regardless of how this is written, it means that the insulin is made up of 70% NPH/isophane insulin and 30% R/neutral insulin.

The most common mixes are 70/30 (30/70) (non analog) or 75/25 (25/75) (analog). There are no currently-marketed mixes which combine insulins of different origins. Eli Lilly's form of beef/pork Iletin and Novo Nordisk's Lentard were discontinued some time ago. Here are the most common ones that could cause confusion:


More Information[]

Related pages
Wp comb50 1
Penfill Mixtard 50 1
Wp comb50 1
Humulin M5
Mixtard 50
Humulin 50/50
Penmix 50