Canine Diabetes Wiki
Humulina 10/90
R/Neutral/NPH/Isophane Mix by Eli Lilly
U100 isophane
Action in dogs:
  • onset 0.5-3h,
  • peak 2-10h,
  • duration 4-24h
Line: Humulin
Also known as: Huminsulin Profil I, Humulin 10/90
Humuline 10/90, Humulin M1, Profil I
Similar to: Actraphane 10/90, Penmix 10 [1], Mixtard 10

Names of Lilly r-DNA/GE/GM insulins worldwide
Use and Handling:
Shelf Life: 24 months Type: cloudy
When Opened: 28 days room temp.
In Pen: 28 days room temp.
Notes: Protect from light and heat
Do Not Freeze, Resuspend
Do not use if product does not re-suspend
Do not use intravenously [2]
10 90

Time activity profile for 10/90 (90/10) insulins.

This is an intermediate-acting, mixed insulin. Humulin 10/90, also made by Eli Lilly, was available in North America. More names for this insulin are Huminsulin Profil I, Humuline 10/90 and Humulin M1. All are similar to Mixtard 10 [3] and Penmix 10 consisting of 10% R/neutral insulin and 90% NPH/isophane insulin crystals. [4][5]

Mixtard 10 was discontinued December 31, 2007. [6][7] Humulin 10/90 is also discontinued.

Mixed insulins cover a wide range as they can be animal origin, r-DNA/GE/GM origin or analog insulins. The non-analog mixed insulins are made up of intermediate-acting NPH/isophane insulin and short-acting R/neutral. normal insulin. The analog mixes are made from intermediate-acting protamine-suspended analog insulin (suspended similar to NPH/isophane insulin) and fast-acting analog insulin. They can also vary in the fraction (amount or percent) of the two types (R/neutral and NPH/isophane) of insulin used to make a non-analog mix, and in the percentage of fast/rapid-acting analog insulin and slower-acting protamine suspended analog insulin.

For some reason, those in the US choose to list the slower, NPH/isophane based insulin fraction first, such as Humulin or Novolin 70/30. The rest of the world gives the faster insulin top billing and the result is 30/70. Regardless of how this is written, it means that the insulin is made up of 70% NPH/isophane insulin and 30% R/neutral insulin.

The most common mixes are 70/30 (30/70) (non analog) or 75/25 (25/75) (analog). There are no currently-marketed mixes which combine insulins of different origins. Eli Lilly's form of beef/pork Iletin and Novo Nordisk's Lentard were discontinued some time ago. Here are the most common ones that could cause confusion:


  1. Penmix 10. Medsafe NZ.
  2. Maddison, Jill E.,Page, Stephen W.,Church, David B. (2008). Small Animal Clinical Pharmacology. Saunders Ltd..
  3. Prescribing Novo Insulins.
  4. Scientific Discussion-Mixtard. EMEA.
  5. Humulin 10/90.
  6. Insulin discontinuations-UK. NHS.
  7. Chart of Discontinued Novo Human Insulins. Novo Nordisk.

Related Pages
10 90 Penfill Mixtard 10 1 10 90 10 90 10 90
Penmix 10
Mixtard 10
Humulin 10/90
Humulin M1
Actraphane 10/90