Canine Diabetes Wiki
Humulin M3
R/Neutral/NPH/Isophane Mix by Eli Lilly
U40, U100 isophane
Action in dogs:
  • onset 0.5-3h,
  • peak 2-10h,
  • duration 4-24h
Line: Humulin
Also known as: Huminsulin Profil III, Humulin 3/7
Humulin 30/70, Humulina 30/70, Humuline 30/70
Profil 30,  Umuline Profil 30, Humulin 70/30
Humulin Mix 30/70, Humulin ReliOn 70/30
Similar to: Actraphane 30/70, Mixtard 30, Penmix 30,
Novolin 70/30, Novolin 30R, ReliOn/Novolin 70/30

Insuman Comb 30, Winthrop Comb 30

Names of Lilly r-DNA/GE/GM insulins worldwide

Use and Handling:
Shelf Life: 24 months Type: cloudy
When Opened: 28 days room temp.
In Pen: 28 days room temp.
Notes: Protect from light and heat
Do Not Freeze, Resuspend
Do not use if product does not re-suspend
Do not use intravenously [1]

Humulin M3-an intermediate-acting insulin consisting of 30% R/Neutral insulin and 70% NPH/Isophane insulin.

Note that some countries have the brand name in U40 strength.

Humulin M3 is the UK brand name for Lilly's r-DNA/GE/GM human insulin mix [2][3][4] consisting of 30% R/neutral insulin and 70% NPH/isophane crystal [5] insulin.

It is the same as Humulin 70/30, [6] Umuline Profil 30, Huminsulin Profil III, Humuline 30/70, Humulina 30/70, Mixtard 30 [7][8][9], ReliOn/Novolin 70/30 and Actraphane 30/70. [10] It's also similar to the Aventis insulins Insuman Comb 30 and Winthrop Comb 30.

Why Lente doesn't equal 70/30[]

Lente-70 30

Direct comparison of insulin activity profiles for Lente and 70/30 insulins. The early strong action from the non-suspended R/neutral/normal insulin is seen at the "bump" from hours 0-6.

Let's look at the differences in the two insulins. Lente is comprised of 70 % long-acting Ultralente insulin and 30% short-acting Semilente insulin. So proportions of a long-acting and short-acting insulin are being combined to produce a intermediate-acting insulin. Both Ultralente and Semilente insulin are suspended by adding zinc and the size of their respective insulin crystals. The largest insulin crystals are those of Ultralente, while Semilente contains the smallest, or microcrystals. Simply put, you're combining a long insulin and a short insulin to make an intermediate-acting one.[11]

70/30 insulin starts with 70% NPH/isophane insulin, an intermediate-acting insulin which is suspended by protamine. To that, 30% R/neutral/normal insulin, which is short-acting and soluble, meaning there is no suspension; nothing is added to this insulin to delay its action. In this case, you are taking an intermediate-acting insulin with a suspension and adding to it a short-acting one with no suspension to create an intermediate-acting insulin.

So we have an insulin (Lente), made up of a long-acting and short-acting insulin, both with zinc suspensions, and a mixed insulin (70/30), made from an intermediate-acting protamine suspended insulin and a short-acting one without any suspension. Because of these differences, the insulin action profile for these two insulins is going to be quite different, even though both insulins are classed as intermediate-acting.

Mixed insulins cover a wide range as they can be animal origin, r-DNA/GE/GM origin or analog insulins. The non-analog mixed insulins are made up of intermediate-acting NPH/isophane insulin and short-acting R/neutral. normal insulin. The analog mixes are made from intermediate-acting protamine-suspended analog insulin (suspended similar to NPH/isophane insulin) and fast-acting analog insulin. They can also vary in the fraction (amount or percent) of the two types (R/neutral and NPH/isophane) of insulin used to make a non-analog mix, and in the percentage of fast/rapid-acting analog insulin and slower-acting protamine suspended analog insulin.

For some reason, those in the US choose to list the slower, NPH/isophane based insulin fraction first, such as Humulin or Novolin 70/30. The rest of the world gives the faster insulin top billing and the result is 30/70. Regardless of how this is written, it means that the insulin is made up of 70% NPH/isophane insulin and 30% R/neutral insulin.

The most common mixes are 70/30 (30/70) (non analog) or 75/25 (25/75) (analog). There are no currently-marketed mixes which combine insulins of different origins. Eli Lilly's form of beef/pork Iletin and Novo Nordisk's Lentard were discontinued some time ago. Here are the most common ones that could cause confusion:


More Information[]

Related pages
NVN18370 73077 5
30 70
30 70
Novolin 70/30
Mixtard 30
Humulin 70/30
Humulina 30/70
Insuman Comb 30