Canine Diabetes Wiki
FDA Warning Letter to Eli Lilly re: instability of insulin Lispro (Humalog) crystals-10 February 2010
Reuters News story re: Lilly/Humalog Warning Letter-23 February 2010
Humalog 75 25

Humalog Mix 75/25, known also as Humalog Mix 25.

Eli Lilly brand name for their mix of 25% insulin lispro and 75% insulin lispro protamine suspension. [1] Also known as Humalog Mix 25, it is an intermediate-acting insulin. The 75% fraction that is NPL (Neutral Protamine Lispro) [2] is a suspension of crystals formed by combining insulin lispro and protamine.

A study of dogs from 1993-1994 using various fractions of Humalog mixes against human NPH/isophane insulin found only minor differences in the use of Humalog Mix as opposed to NPH/isophane insulin. [3]

Analog Insulins: amino acid sequences[]

Analog Insulin pharmacokinetics[]

These are human activity profiles.


  1. Humalog 75/25-Insulin Lispro Protamine; Insulin Lispro. Revolution Health.
  2. Humalog 75/25 patient leaflet. Eli Lilly.
  3. Humalog Pharmacology Review-Pages 5 & 6. US Food and Drug Administration.
  4. 4.0 4.1 Guide to Insulin Preparations. Pharmacy Times.
  5. 5.0 5.1 Insulin Pharmacology. Cite error: Invalid <ref> tag; name "Endotext" defined multiple times with different content
  6. Hypurin Protamine Zinc.

More Information[]