Canine Diabetes Wiki
Betasint Bovine NPH
NPH/isophane by Beta Laboratorios
U40, U80, U100 isophane
Action in dogs:
  • onset within 2h,
  • peak 6-12h,
  • duration 18-14h
Line: Betasint
Also known as: Beef NPH, beef isophane
Similar to: Hypurin Bovine Isophane, Iletin NPH
Use and Handling:
Shelf Life: 36 months Type: cloudy
When Opened: 28 days room temp.
In Pen: 28 days room temp.
Notes: Protect from light and heat
Do Not Freeze, Re-suspend
Do not use if product does not re-suspend

Cartridges in use are not to be refrigerated
Do not use intravenously [1]
May be used intramuscularly, which means onset, peak and duration times are shorter.
Store between 2-8C,
May be mixed with Bovine Neutral


Betasint logo. Bovine insulin was successfully extracted in January 1987-porcine followed in June of the same year.

This is an intermediate-acting beef isophane insulin. Betasint Bovine NPH [2][3] is comparable to Hypurin Bovine Isophane. [4][5][6]

A comparable product, formerly made by Eli Lilly was all-beef Iletin I NPH.

Specifications of NPH/isophane insulin[]

British National Formulary [7] defines this type as: A sterile suspension bovine or porcine or of human insulin in the form of a complex obtained by the addition of protamine sulphate or another suitable protamine.

British Pharmacoepia and United States Pharmacopia definitions: [7]

Isophane Insulin

Sterile buffered suspension of insulin in the form of a complex obtained by addition of suitable protamine. Prepared from crystalline insulin. pH 6.9 - 7.5 iso-osmotic with blood. Contains for each 100 units of insulin, 300 - 600 g protamine sulphate and not more than 40 g zinc, a suitable bactericide and sodium phosphate as buffering agent.

USP specification: Sterile suspension of zinc insulin crystalline and protamine sulphate in buffered water for injection. Solid phase contain crystals of insulin protamine and zinc; 40, 80, 100 units/ml. Contains glycerol, metacresol, phenol sodium phosphate and zinc.

NPH/isophane insulins[]

The following commercial insulins are all generically NPH insulins:

Other commonly used names are:

  • NPH insulin, NPH
  • isophane insulin


  1. Maddison, Jill E.,Page, Stephen W.,Church, David B. (2008). Small Animal Clinical Pharmacology. Saunders Ltd..
  2. Betasint Bovine NPH. Beta Laboratorios.
  3. Google translation of Betasint Bovine NPH page.
  4. 4.0 4.1 Hypurin Bovine Isophane Patient Information. Net Doctor UK.
  5. Hypurin Bovine Isophane. Wockhardt UK.
  6. 6.0 6.1 Prescribing Bovine Insulin.
  7. 7.0 7.1 British National Formulary-Isophane/NPH Insulin Definition. InChem. Cite error: Invalid <ref> tag; name "InChem" defined multiple times with different content
  8. Hypurin Bovine Isophane. Electronic Medicines Compendium UK.
  9. 9.0 9.1 Response to Withdrawal of Novo Porcine Insulins. CP/Wockhardt UK.
  10. Prescribing Porcine Insulin.
  11. Prescribing Novo Animal Insulin.
  12. Patient Information Leaflet-Pork Insulatard. Novo Nordisk.

Related pages
Lilly human insulins1992charts
Cp wockhardt bovine insulins
Iletin NPH
Hypurin Bovine Isophane