Canine Diabetes Wiki
Actraphane 50/50
R/Neutral/NPH/Isophane Mix by Novo Nordisk
U40, U100 isophane
Action in dogs:
  • onset 0.5-3h,
  • peak 2-10h,
  • duration 4-24h
Line: Novo
Also known as: Mixtard 50, Penmix 50
Similar to: Huminsulin 50/50,Humulin M5
Humulin 50/50,Humulina 50/50, Humuline 50/50
Insuhuman Comb 50, Insuman Comb 50
Winthrop Comb 50

Names of Lilly r-DNA/GE/GM insulins worldwide
Use and Handling:
Shelf Life: 30 months Type: cloudy
When Opened: 6 weeks room temp.
In Pen: 6 weeks room temp.
Notes: Protect from light and heat
Do Not Freeze, Resuspend
Do not use if product does not re-suspend
Do not use intravenously [1]

50 50

Time activity profile for Actraphane 50/50.

A 50% R/neutral and 50% NPH/isophane crystal mixed insulin [2] that's intermediate-acting by Novo Nordisk.

Comparable insulins are: Humuline 50/50, Humulina 50/50, Humulin M5, Humulin 50/50 [3][4], Mixtard 50 [2][5][6], Winthrop Comb 50 and Insuman Comb 50. [7][8] Eli Lilly produced an identical insulin under the name Humulin M5 [9] in the EU & UK; it was withdrawn in July 2004 from those markets. [10]

Mixed insulins cover a wide range as they can be animal origin, r-DNA/GE/GM origin or analog insulins. The non-analog mixed insulins are made up of intermediate-acting NPH/isophane insulin and short-acting R/neutral. normal insulin. The analog mixes are made from intermediate-acting protamine-suspended analog insulin (suspended similar to NPH/isophane insulin) and fast-acting analog insulin. They can also vary in the fraction (amount or percent) of the two types (R/neutral and NPH/isophane) of insulin used to make a non-analog mix, and in the percentage of fast/rapid-acting analog insulin and slower-acting protamine suspended analog insulin.

For some reason, those in the US choose to list the slower, NPH/isophane based insulin fraction first, such as Humulin or Novolin 70/30. The rest of the world gives the faster insulin top billing and the result is 30/70. Regardless of how this is written, it means that the insulin is made up of 70% NPH/isophane insulin and 30% R/neutral insulin.

The most common mixes are 70/30 (30/70) (non analog) or 75/25 (25/75) (analog). There are no currently-marketed mixes which combine insulins of different origins. Eli Lilly's form of beef/pork Iletin and Novo Nordisk's Lentard were discontinued some time ago. Here are the most common ones that could cause confusion:


  1. Maddison, Jill E.,Page, Stephen W.,Church, David B. (2008). Small Animal Clinical Pharmacology. Saunders Ltd..
  2. 2.0 2.1 Scientific Discussion Actraphane. EMEA. Cite error: Invalid <ref> tag; name "EMEA" defined multiple times with different content
  3. Humulin 50/50;NPH;R Human Insulin.
  4. Insulin Chart-Page 2. NACDS.
  5. Insulins & Mixtures. NHS-UK.
  6. Prescribing Novo Insulins.
  7. Patient information Leaflet-Insuman Comb 50. Sanofi Aventis.
  8. Prescribing Insuman Insulins.
  9. Lilly EU/UK Product Names & Time Activity Profiles. Eli Lilly.
  10. Withdrawal of Humulin M5-. Pharmaceutical Journal (July 2004).
Related pages
Humulin M5
Penfill Mixtard 50 1
Wp comb50 1
Humulin M5
Mixtard 50
Humulin 50/50
Penmix 50